Specimen for total cholesterol should be fasting serum (pt fasting for 12 hours)
Method for total cholesterol is part of four different lipoproteins; to measure total cholesterol, must take all lipoproteins and break them apart to free up cholesterol
cholesterol assay measures cholesterol in chylomicrons, VLDL, IDL, LDL, HDL
Triglycerides tend to be diet dependent
Specimen for HDL/LDL- fasting serum
hdl and total cholesterol use same methodology
bruh
A) cholesterol ester
B) cholesterol esterase
C) cholesterol
D) FA
E) cholesterol
F) O2
G) cholesterol oxidase
H) cholestenone
I) H2
J) O2
K) H2O2
L) dye
M) peroxidase
N) color
O) H2O
Lecithin/Sphingomyelin ratio - done on amniotic fluid to test for fetal lung maturity
Done via thin layer chromatography
Fecal fat - 72 hour specimen
Many pts doing this are supposed to be on high lipid diet
