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Learning Objectives
assays
assays for urea
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Cards (2)
GLDH coupled enzymatic reaction
A)
urea
B)
H2o
C)
urease
D)
2NH4+
E)
CO32-
F)
NH4+
G)
2-oxoglutarate
H)
NADH
I)
GLDH
J)
NAD+
K)
glutamate
L)
H2o
12
diacetyl monoxide assay
from
colorless
to
yellow
A)
diacetyl monoxime
B)
h2o
C)
H+
D)
diacetyl
E)
HONH2
F)
urea
G)
diacetyl
H)
heat, H+
I)
diazene (yellow)
J)
2H2o
10