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• spectrophotometry uses light absorbance at specific wavelength to determine analyte concentration
• wavelength is also called lambda
• wavelength can be measured from peak to peak or from trough to trough
• wavelength is inversely related to frequency
• the longer the wavelength, the lower the frequency
• the shorter the wavelength, the higher the frequency
• the visible range of light is 400 - 700 nm
• number of waves emitted goes down on either side of target wavelength