Investigating enzymatic reactions

• What does amylase catalyze?


  Amylase catalyzes the conversion of starch to maltose.
• What is the purpose of the experiment described in the study material?

  • Investigate how pH affects amylase activity
  • Measure reaction time for starch breakdown
• What is the first step in the experimental procedure for investigating enzymatic reactions?


  Add iodine solution into every well of a spotting tile.
• What equipment is used to heat the water in the experiment?

  A Bunsen burner is used to heat the water.
• Why is it important to keep the temperature constant at 35°C during the experiment?


  To ensure consistent enzyme activity and accurate results.
• How much amylase solution is added to the boiling tube?


  10 mL of amylase solution is added.
• What is the next step after adding the amylase and buffer solution to the tube?


  Add 5 mL of starch solution to the tube.
• What should be done every 30 seconds during the experiment?


  Take a fresh sample from the tube and place it in a well.
• What indicates that the starch is no longer present in the reaction?


  The sample turns orange when starch is no longer present.
• What should be done after completing the first trial of the experiment?


  Repeat the experiment with different pH solutions.