Microscopy

• What is the unit conversion for 1 kilogram in grams?


  1 kg = 1000 g
• How many centimeters are in 1 meter?

  1 cm = 100 cm
• How many millimeters are in 1 centimeter?


  1 cm = 10 mm
• How many micrometers are in 1 millimeter?


  1 mm = 1000 µm
• How many nanometers are in 1 picometer?


  1 pm = 1000 nm
• What is the maximum magnification of a light microscope?


  2000 times
• When was the electron microscope invented?


  1936
• What is the primary function of an electron microscope?


  To view subcellular structures
• How does an electron microscope form an image?


  By using a beam of electrons
• What is the maximum magnification of an electron microscope?


  2000000 times
• What is magnification in microscopy?


  Magnification is the ratio of image size to real object size
• If the eyepiece lens magnification is $4$ and the objective lens magnification is $40$, what is the total magnification?


  $160$
• What does resolution in microscopy refer to?


  Resolution is the ability to distinguish between two separate points
• How does resolving power affect detail in microscopy?


  Higher resolving power allows for more detail to be seen
• What is the method for preparing onion cells for observation under a light microscope?


  1. Place specimen on a microscope slide.
  2. Add a drop of water to the middle.
  3. Cut up onion and separate its layers.
  4. Peel off epidermal tissue using tweezers.
  5. Place tissue into the water on the slide.
  6. Add a drop of iodine solution to stain.
  7. Place a cover slip on top.
• What are the steps to use a light microscope effectively?


  1. Clip slide onto the stage.
  2. Select the lowest-powered objective lens.
  3. Use coarse adjustment knob to move stage up.
  4. Look down eyepiece and adjust until focused.
  5. Swap to higher-powered objective lens for higher magnification.
• What is the purpose of adding iodine solution to the specimen?


  To stain and add color to highlight the specimen
• What should be avoided when placing the cover slip on the slide?


  Air bubbles
• What is the function of the coarse adjustment knob on a microscope?


  To move the stage up and down for focusing
• What is the function of the fine adjustment knob on a microscope?


  To make precise adjustments to focus
• What are the components of a light microscope?


  Eyepiece, coarse adjustment knob, fine adjustment knob, objective lenses, stage, light